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| Registros recuperados: 13 | |
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| Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed... |
| Tipo: Text |
Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf |
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| Figueiredo,José Altair; Andrade Júnior,Valter C de; Pereira,Rosana Cristina; Ribeiro,Karina G; Viana,Daniel José S; Neiva,Irã P. |
| O presente trabalho teve como objetivo selecionar clones de batata-doce com potencial para uso na alimentação animal. O experimento foi conduzido em blocos ao acaso, com 11 clones e quatro repetições. Os clones avaliados fazem parte do banco de germoplasma da Universidade Federal dos Vales do Jequitinhonha e Mucuri, sendo: BD-06, BD-25, BD-15, BD-38, Cambraia, BD-31-TO, BD-67, BD-45, BD-42, BD-54 e a cultivar Brazlândia Rosada. Nas ramas coletadas do experimento foram avaliados o teor de matéria seca e as produtividades de massa verde e massa seca. Nas silagens de ramas foram avaliados o pH e os teores de matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), nutrientes digestíveis totais (NDT), fósforo,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipomoea batatas; FDA; FDN; NDT; MS; Proteína bruta. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000400024 |
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| Ribeiro,L.P.; Freitas-Lima,L.C.; Naumann,G.B.; Meyrelles,S.S.; Lunz,W.; Pires,S.F.; Andrade,H.M.; Carnielli,J.B.T.; Figueiredo,S.G.. |
| In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inborn aerobic capacity; Proteomics; 2-DE; MS; Heart proteins. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609 |
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| Villa,Fabíola; Pasqual,Moacir; Pio,Leila Aparecida Salles; Assis,Franscinely Aparecida; Zárraga,Danielle Zampiere Arce. |
| A fim de aprimorar técnicas de cultivo in vitro de duas frutíferas de clima temperado (amoreira-preta cv. Tupy e porta-enxerto de videira cv. Kobber 5BB), testaram-se diferentes concentrações de glicina e inositol, adicionadas ao meio de cultura. Para plântula de amoreira-preta, o meio foi constituído do meio básico MS, acrescido de 30 g L-1 de sacarose e 7 g L-1 de ágar, e o pH ajustado para 5,8 antes da autoclavagem a 121ºC e 1 atm por 20 minutos; e do meio básico DSD1 para porta-enxerto de videira cv. Kobber 5BB, acrescido de 20 g L-1 de sacarose e 7 g L-1 de ágar, e o pH ajustado para 6,4. O experimento com amoreira-preta consistiu de 5 diferentes concentrações de glicina (0; 1,0; 2,0; 4,0 e 8,0 mg L-1), 5 de inositol (0; 50; 100; 200 e 400 mg L-1) e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitamina; Aminoácido; MS; DSD1; Amoreira-preta; Videira. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000500042 |
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| Longo, Sébastien; Sibat, Manoella; Taiana Darius, Hélène; Hess, Philipp; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21,... |
| Tipo: Text |
Palavras-chave: Gambierdiscus polynesiensis; French Polynesia; Ciguatera; Ciguatoxins; LC-MS; MS; Toxin profile; Nitrate; Urea; Culture medium acidification. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77382/78981.pdf |
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| Campêlo,José Elivalto Guimarães; Oliveira,José Crisóstomo Gomes de; Rocha,André da Silva; Carvalho,José Francisco de; Moura,Geyson Coutinho; Oliveira,Maria Elizabete de; Silva,José Algaci Lopes da; Moura,José Wilson da Silva; Costa,Valter Marchão; Uchoa,Lindomar de Morais. |
| Esta pesquisa foi realizada visando avaliar a qualidade do volumoso produzido com a técnica de hidroponia. Como substratos, utilizaram-se casca de arroz e capim-elefante picado cultivado sobre lona plástica, com densidade de 2,5 kg de grãos/m². Os dados foram analisados em delineamento inteiramente casualizado, com sete repetições, representadas por canteiros de 0,5 x 0,5 m de área útil. A fertiirrigação foi usada do 3º ao 14º dia após a semeadura, quando foi realizada a colheita para determinação da produtividade da forragem natural/m², da porcentagem de MS total e dos teores de PB, FDN, FDA e cinzas. Essas características foram determinadas em amostras da forragem completa, do substrato + raízes e em amostras apenas das plantas. O uso de casca de arroz... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alimento alternativo; FDN; FDA; MS; PB; Ruminantes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000200002 |
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| Tavares,Armando R; Young,Jorge Luiz M; Ori,Sandra S; Kanashiro,Shoey; Lima,Giuseppina PP; Chu,Edison Paulo; Suzuki,Rogério M. |
| In vitro cultivation is the main propagation method for the family Orchidaceae, whereas nitrogen is the most important nutrient in the culture media. This work was carried out to study the influence of different nitrogen concentrations on the in vitro growth of the orchid Phalaenopsis amabilis. Nitrogen concentrations varied by altering the ionic balance of the Murashige & Skoog (MS) culture medium. Plants, 360 days old, were cultivated in liquid MS, modified with 7.5, 15, 30, 45, and 60 mM N. After 180 days, we assessed plant and root length, number of leaves and roots, and fresh and dry weight of leaves, roots and plants. Treatments were assigned to completely randomized plots, with four replications. Plots consisted of five three-plant flasks.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phalaenopsis amabilis; MS; Mineral nutrition; Nitrogen. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000100020 |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tectus niloticus; Gastropods; Gambierdiscus polynesiensis; QPCR assays; CBA-N2a; LC-MS; MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00426/53798/54719.pdf |
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| Estevez, Pablo; Sibat, Manoella; Leão-martins, José Manuel; Tudó, Angels; Rambla-alegre, Maria; Aligizaki, Katerina; Diogène, Jorge; Gago-martinez, Ana; Hess, Philipp. |
| Over the last decade, knowledge has significantly increased on the taxonomic identity and distribution of dinoflagellates of the genera Gambierdiscus and Fukuyoa. Additionally, a number of hitherto unknown bioactive metabolites have been described, while the role of these compounds in ciguatera poisoning (CP) remains to be clarified. Ciguatoxins and maitotoxins are very toxic compounds produced by these dinoflagellates and have been described since the 1980s. Ciguatoxins are generally described as the main contributors to this food intoxication. Recent reports of CP in temperate waters of the Canary Islands (Spain) and the Madeira archipelago (Portugal) triggered the need for isolation and cultivation of dinoflagellates from these areas, and their... |
| Tipo: Text |
Palavras-chave: Maitotoxins; Ciguatoxins; Gambierdiscus; Fukuyoa; LC-MS; MS; HRMS; QToF; Ciguatera poisoning. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74081/73530.pdf |
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| Registros recuperados: 13 | |
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